For more than 10 years Tyler has used his experience in smart home tech to craft how-to guides, explainers, and recommendations for technology of all kinds. From using his home in beautiful Bend, OR ...
1| Incubate the cell lysate with 50 μl of a 50% slurry of glutathione-agarose beads and 25 μg of GST for 2 h at 4 °C with end-over-end mixing. The amount of lysate needed to detect an interaction is ...